Precision tissue staining every time
at the push of a button

How it works

Greater workflow efficiency - saves time and reduces the need for dedicated personnel and training - automated liquid handlers are faster than manual pipetting.

making tissue auto-staining accessible to all

Peace of mind of having reproducible results – with simple or complex protocols

Omni-Stainer™ C12

for coverslips

  • Compact: footprint of a 96-well plate
  • Stains up to 12 coverslip-mounted samples at a time
  • Saves reagents: 50% less reagents compared to open-top staining
  • Never lose or break a sample
  • Built-in wet chamber
  • Use it by hand or insert into any SBS-compatible liquid handler to automate your staining
Omni-stainer-C12-with-tip-open-lid-3

How it works

for coverslips

how-it-works-coverslips
  1. Coverslip with sample rests on 50um thin spacers, forming a capillary chamber. Staining reagents are supplied by a liquid handler (or by hand!)
  2. Sample is incubated in a thin capillary layer
  3. When the sample is washed, the old one is instantly displaced by the laminar flow
Omni-stainer-S12-with-samples

Omni-Stainer™ S12

for slides

  • Compact: footprint of a 96-well plate
  • Stains up to 12 coverslip-mounted samples at a time
  • Saves reagents: 50% less reagents compared to open-top staining
  • Never lose or break a sample
  • Built-in wet chamber
  • Use it by hand or insert into any SBS-compatible liquid handler to automate your staining

How it works

for slides

how-it-works-for-slides
  • The same capillary displacement principle, but the sample is mounted on a slide and covered with a covertile that incorporates capillary spacers

EFFICIENT - ACCURATE - AFFORDABLE

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Virtual Demo

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Placing slides into Omni-Stainer™ 

Slide assembly
This video shows how to assemble the slide/covertile sandwich and place it into the slide Omni-StainerTM. First the tacky spacers are exposed by removing the protective tape from the covertile. To avoid airbubbles between the slide and the covertile the slide is first placed in the small tray with compatible buffer (water or PBS) and in such submerged stage is covered with a covertile. The spacers are tacky enough to hold the covertile in place (after slightly pressed against the slide) but yet when wet allow repositioning the covertile over the slide. After the sandwich is assembled the combo is placed on the shelf and then into the Omni-StainerTM

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Coverslip Assembly
This video shows how to assemble the coverslip/coverslip pad sandwich and place it into the coverslip Omni-StainerTM To avoid airbubbles between the slide and the covertile a drop of compatible buffer (water or PBS) is first placed onto the coverslip pad. The coverslip with a sample is then placed face down on the pad to avoid bubble formation and ensure that the liquid fully fills the flow cell formed by sandwiching the coverslip pad and the coverslip. After the sandwich is assembled the combo is placed into the coverslip version of the Omni-StainerTM.

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Manual staining

Using Omni-Stainer™ by hand

Play Video

Omni-Stainer™ S12 Demo Video

Play Video

Omni-Stainer™ C12 Demo Video

The Omni-StainerTM can be used as a device for manual staining. In such case the solutions are exchanged by manual pipetting. After the pipetting the Omni-StainerTM should be closed with a lid. In such configuration it can be placed into the 4C refrigerator or into the heated incubator. Before the start of any staining experiment 20-30ml of water should be placed into the Omni-StainerTM to create the humid chamber environment which helps to reduce the evaporation of staining solutions from the slide/covertile or coverslip/pad sandwich.”

Automated Staining Demo

using Opentrons OT-2

Play Video

Omni-Stainer™ S12 automation demo

Play Video

Omni-Stainer™ C12 Demo Video

For automated liquid exchange the omnistainer has to be place into programmable liquid handler (e.g. Opentrons). The design of the Omni-StainerTM lid including the prewetted perforated filter paper on the lid ensures minimizing the evaporation of staining reagents from the slide/covertile or coverslip/pad sandwich.

Liquid handling

OT-2

Staining automation can be attained by inserting the Omni-Stainer™ into an SBS-compatible liquid handler, and how we are partnering with Opentrons and adopting their versatile ultra-low-cost liquid robot OT-2 to deliver complete staining automation solutions.

As a part of our ‘automation package’, we provide pre-written protocols for various assays, including H&E, IHC, IF, CycIF, CODEX, FFPE antigen retrieval, CAS-FISH, IMC, RNAscope. Custom protocol development services are available.

Omni-Stainer™ is compatible with most liquid handling robots but if you don't have one, we recommend Opentrons OT-2

Protocols Supported on OT-2:

  • IF
  • IHC
  • CODEX single and multi-strain
  • Cas-FISH
  • FFPE Antigen Retrieval (HIER)
  • Opal

Coming soon:

  • smRNA-FISH
  • Rolling Circle Amplification
  • Cyclic IF
  • IMC & MIBI
  • and many more...
OT 2

Heating and cooling on the robot: FFPE-IHC

Omni-Stainer™ rests on top of the OT-2 thermal module after:
Human tonsil FFPE antigen retrieval at 95°C
Anti-CD8 Primary antibody staining at 4°C
Secondary antibody staining, DAB and hematoxylin at room temperature

Heating and cooling on the robot: FFPE-IHC

Are you ready for Omni-Stainer™ to transform your life?

What people are saying...

With the Parhelia Omni-Stainer I’ve been able to increase the number of coverslips or slides I can do CODEX staining at the same time and, on top of that, I’ve been able to save reagents and antibody while preserving staining quality. Using their product was a great investment for our project

Ruan Medrano, Ph.D

Washington University in St. Louis School of Medicine

We were very excited to be an early-access adopter of Parhelia’s automated coverslip staining solution. Their product significantly improved the reproducibility, labor, and throughput of our smFISH and CasFISH workflows. The Parhelia team provided easy to implement software scripts to run the protocols on the OT-2, and were very responsive in answering any of our questions as we were first setting up the process. Finally, the low upfront capital acquisition cost versus other cell staining automation solutions makes it very easy to justify the ROI.

Un Kwon-Casado

Dahlia Biosciences - Co-Founder and CEO

I am not so sure about this Parhelia thing… I’d rather buy a machine from a big vendor that takes up the whole room, guzzles gallons of proprietory antibodies per run and costs 50X more.

Said Nobody

Ever

Testimonial 1