This protocol allows for sample incubation at  room temperature.  Staining reagents are aspirated from a 96-well plate and buffers from a 12-trough reservoir.  Staining reagents are placed in order, starting in column one and are subsequently added for each additional sample.  Each well requires 130 μl of reagent.  10 μl of fixative is added to the wells in row C by the user upon set up.  This will be mixed with 150 μl of PBS by the PSS, right before it’s added to the sample

This protocol allows for room temperature incubation of all reagents, as the thermal module is not in use.  2.0 mL tubes are held in a 24 microtube rack which contain all non-buffer reagents, including the amplification reagents.  These amp reagents go into six separate tubes, each designated for an amplification cycle.  This protocol is for one round of staining.  After completion, samples are imaged and then the same protocol can be used for the second round of staining.

This protocol aspirates  staining reagents from from a 96-well plate and buffers from a 12-trough reservoir.  All samples sit on the temperature module to allow for a 14 hour antibody incubation at 6°C.  Staining reagents are placed in order, starting in column one and are subsequently added for each additional sample.  Each well requires 130 μl of reagent.